Enzymes Make the World Go 'Round We have a whole section where we tell you about reactions and the molecules that change in those reactions. Chemical bonds are being created and destroyed over a series of many intermediate reactions. Those changes rarely happen on their own when you look at biological systems.
Polymerase chain reaction (PCR) is an efficient and cost-effective molecular tool to copy or amplify small segments of DNA or RNA. PCR combines the principles of complementary nucleic acid hybridization with those of nucleic acid replication that are applied repeatedly through numerous cycles.
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In this reaction, the second step is the rate-limiting step. No matter how fast the first step takes place, the overall reaction cannot proceed any faster than the second step in the reaction. As we have seen, the rate of any step in a reaction is directly proportional to the concentrations of the reactants consumed in that step.

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